School of Biotechnology

Madurai Kamaraj University, Madurai



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   K.Veluthambi
   Professor
   Dept.of Plant Biotechnology
   kveluthambi@rediffmail.com
  

Research Publication

Research Interest:

  •  Study of Mechanism of Agrobacterium T-DNA transfer and improvement of Ti plasmid vector systems: 'Long transfer' effects in Agrobacterium Ti plasmid vectors and vir gene induction by rice have been demonstrated.

  •  Cloning and analysis of Mungbean yellow mosaic virus:- Cloning and sequencing of the virus have been achieved. Transgenic rice plants generated with Chitinase, B-1, 3-glucanase, osmotin and thaumatin - like protein genes exhibit high levels of sheath blight resistance.
     
  •  Genetic engineering of crop plants for fungal and virus resistance.


Major ongoing research programmes in the lab are:

  1. RESEARCH WORK CARRIED OUT DURING 2000-2005

    1. Genetic Engineering of rice for sheath resistance
    2. Molecular Biology of Mungbean Yellow mosaic virus Vigna(MYMV-Vig) and Genetic Engineering of blackgram for MYMV-Vig resistance.
    3. Genetic Engineering of coffee for leaf rust resistance.
    4. Development of Ti plasmid vector for selection marker elimination in transgenic plants.

    MAJOR RESEARCH RECOGNITIONS DURING 2000-2005

    1. Elected Fellow of Indian Academy of Sciences(2002)
    2. Elected Member-Guha Research Conference

    PLAN FOR RESEARCH WORK DURING 2005 - 2010

    1. Greenhouse and Field trials ot Transgenic rice with sheath blight resistance.
    2. Genetics Engineering of Blackgram for MYMV-Vig resistance.
    3. Rice functional genomics - 'Gene knock out' in rice.
    4. Marker-free transgenic rice-Technology Development.


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RESEARCH PUBLICATIONS (1994 � 2005)

    1. Shivaprasad, P.V., R. Akbergenov, D. Trinks, R. Rajeswaran, K. Veluthambi, T. Hohn and M. M. Pooggin. 2005. Promoters, transcripts and regulatory proteins of mungbean yellow mosaic geminivirus. J. Virology 79: 8149-8160.
    1. Guerra-Peraza, O., D. Kirk, V. Seltzer, K. Veluthambi, A.C. Schmidt, T. Hohn and E. Herzog.  2005.  Coat proteins of Rice tungro bacilliform virus and Mungbean yellow mosaic virus contain multiple nuclear-lacalization signals and interact with importin a.  J. General Virology 86: 1815-1826.
    1. Trinks, D., R. Rajeswaran, P. V. Shivaprasad, R. Akbergenov, E.J. Oakeley, K. Veluthambi, T. Hohn and M.M. Pooggin. 2005.  Suppression of RNA silencing by a geminivirus nuclear protein, AC2, correlates with transactivation of host genes.  J. Virology 79: 2517-2527.
    1. Sridevi G.,  M.Dhandapani and K.Veluthambi.  2005.  Agrobacterium-mediated transformation of White Ponni,  a non-basmati variety of indica rice (Oryza sativa L.).  Current Science  88: 128-132.
    1. Karthikeyan, A.S., R. Vanitharani, V. Balaji, S. Anuradha, P. Thillaichidambaram, P. V. Shivaprasad, C. Parameswari, V. Balamani, M. Saminathan and K. Veluthambi.  2004.  Analysis of an isolate of Mungbean yellow mosaic virus (MYMV) with a highly variable DNA B component.  Archives of Virology 149: 1643-1652. 
    1. Balaji, V., R. Vanitharani, A.S. Karthikeyan, S. Anbalagan and K.Veluthambi.  2004.  Infectivity analysis of two variable DNA B components of Mungbean yellow mosai virus �Vigna in Vigna mungo and  Vigna radiata. Journal of Biosciences 29: 297-308.
    1. Sridevi, G., N. Sabapathi, P. Meena, R. Nandakumar, R. Samiyappan, S. Muthukrishnan, and K. Veluthambi.  2003.  Transgenic indica rice variety Pusa Basmati 1 constitutively expressing a rice chitinase gene exhibits enhanced resistance to Rhizoctonia solani.  J. Plant Biochem & Biotechnol 12: 93-101.
    1. Jacob, S.S., R. Vanitharani, A.S. Karthikeyan, Y. Chinchore, P. Thillaichidambaram, and K. Veluthambi.  2003.  Mungbean yellow mosaic virus-Vi agroinfection by codelivery of DNA A and DNA B from one Agrobacterium strain.  Plant Disease 87: 247-251.
    1. Mikhail Pooggin, P.V. Shivaprasad, K. Veluthambi, and Thomas Hohn.  2003.  RNAi targeting of DNA virus in plants.  Nature Biotechnology 21: 131-132.
    1. Veluthambi, K., Aditya K. Gupta and Aurn Sharma.  2003.  The Current status of plant transformation technologies.  Current Science 84: 368-380.
    1. V. Balaji, P. Rajamuni, G. Sridevi and K. Veluthambi.  2003.  Agrobacterium-mediated Transformation Efficiency in Blackgram and Rice Enhanced by Multiple Copies of pTiBo542 virB and virG.  Indian Journal of Biotechnology 2:  138-146.
    1. Subha S. Jacob and K. Veluthambi.  2003.  A Cointegrate Ti Plasmid Vector for Agrobacterium tumefaciens-mediated Transformation of indica Rice cv Pusa Basmati 1.  J. Plant Biochemistry & Biotechnology 12: 01-09.
    1. Jacob, S.S. and  K. Veluthambi.  2002. Generation of selection marker-free transgenic plants by cotransformation of a cointegrate vector T-DNA and a binary vector T-DNA in one Agrobacterium tumefaciens strain.  Plant Science 163: 801-806.
    1. Atmakuri  Krishnamohan,  V.  Balaji  and  K. Veluthambi.  2001.   Efficient vir  gene  induction in Agrobacterium tumefaciens requires virA, virG and vir box from   the same Ti  plasmid.   Journal of Bacteriology 183:  4079-4089.
    1. Saunders, K., C. Wege, K. Veluthambi,  H. Jeske  and J. Stanley (2001).   The  distinct  phenotypes  of  the  common  and  yellow vein strains of  Tomato  golden  mosaic  virus are  determined  by  nucleotide  differences in the 3�-terminal region of the gene encoding the movement protein.  Journal of General Virology 82: 45-51.
    1. Phogat,  S.  K.,    A.  S.  Karthikeyan   and    K. Veluthambi   (1999).    Generation of  transformed  calli  of  Vigna  radiata  (L.)  Wilczek   by Agrobacterium tumefaciens-mediated transformation. Journal of Plant Biology 26: 77-82.
    1. Sunilkumar, G.  K.  Vijayachandra  and  K.  Veluthambi.   1999.    Preincubation of cut tobacco leaf explants  promotes Agrobacterium-mediated transformation by increasing vir gene induction.  Plant Science  141:  51-58.
    1. Madhava Naidu, M.  K. Veluthambi,  C. S. Srinivasan and R. Naidu.  1998.  Agrobacterium-mediated transformation in Coffea canephora.  Developments in     plantation crops Research pp. 46-50.
    1. Mariamma, M., B. Muthukumar,  K. Veluthambi and A. Gnanam.  1997.  Effects of high temperature stress on the expression of low molecular weight heat shock proteins in rice leaves.  Journal of Plant Physiology  151: 763-765.
    1. Muthukumar,  B., M.  Mariamma,  K. Veluthambi and A. Gnanam.   1996.  Genetic transformation of cotyledon explants of cowpea (Vigna unguiculata L. Walp) using Agrobacterium tumefaciens.  Plant Cell Reports 15: 980-985.
    1. Palanichelvam,  K. and K. Veluthambi.  1996.  Octopine- and nopaline-induced proteins in Agrobacterium tumefaciens are also induced by arginine.  Current Microbiology  33:  156-162.
    1. Ramanathan,  Vai.  and K. Veluthambi.  1996.  Analysis of Octopine left border-directed  transfer  from  Agrobacterium  to  plants.   Journal of Biosciences  21:  45-57.
    1. Karthikeyan,  A.S., K. S.  Sarma and K. Veluthambi.  1996.  Agrobacterium tumefaciens-mediated transformation of Vigna mungo (L.)  Hepper.  Plant Cell Reports  15:  328-331.
    1. Vanitha Rani,  R.,  A.S.  Karthikeyan,  S. Anuradha and K. Veluthambi.  1996.  Genome homologies among geminiviruses infecting Vigna, cassava,  Acalypha,  Croton amd Vernonia.  Current Science (India)  70: 63-69
    1. Sarma, K.S.,  G. Sunil Kumar,  V. Balamani and K. Veluthambi.   1995.  Transient b-glucuronidase expression and generation of shoot buds are highly correlated in Agrobacterium transformed tobacco leaf rings.  Plant Molecular Biology Reporter  13: 377-382.
    1. Vijayachandra, K.,  K. Palanichelvam,  and K. Veluthambi.  1995.   Rice scutellum induces Agrobacterium tumefaciens vir genes and T-strand generation.  Plant Molecular Biology  29:  125-133.
    1. Ramanathan,  Vai. and K. Veluthambi.  1995.  Transfer of non T-DNA portions of Agrobacterium  Ti plasmid pTiA6 from the left terminus of TL-DNA.  Plant Molecular Biology  28:  1149-1154.
    1. Veluthambi, K., M. Krishnan,  J.H. Gould, R.H.  Smith and S.B. Gelvin.  1989.  Opines stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti plasmid.  Journal of Bacteriology 171:  3696-3703.
    1. Veluthambi, K., W. Ream and S.B. Gelvin.  1988.  Virulence genes, borders and overdrive generate single-stranded T-DNA molecules from the A6 Ti plasmid of Agrobacterium tumefaciens.   Journal of Bacteriology 170:  1523-1532.
    1. Jayaswal, R.K., K. Veluthambi,  S.B. Gelvin and J.L. Slightom.  1987.  Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in  Escherichia  coli  by a  virD-encoded  border-specific  endonuclease from Agrobacterium tumefaciens.  Journal of Bacteriology  169: 5035-5045.
    1. Veluthambi,  K., R.K. Jayaswal and S.B. Gelvin.  1987.  Virulence genes A, G and D mediate the double-stranded border cleavage of T-DNA from the Agrobacterium Ti plasmid.  Proceedings of the National Academy of Sciences USA  84:  1881-1885.
    1. Veluthambi, K.  and B.W.  Poovaiah.  1986.  In vitro and in vivo protein            phosphorylation in Avena sativa L. coleoptiles:   Effects of Ca2+,  calmodulin antogonists and auxin.  Plant Physiology  81:  836-841.
    1. Balamani, V., K. Veluthambi and B.W. Poovaiah.  1986.  Role of calcium in the control of tuberization in potato (Solanum tuberosum L.).  Plant Physiology 80: 856-858.
    1. Raghothama, K.G.,  K. Veluthambi and B.W. Poovaiah.  1985.  Stage-specific changes in calcim-regulated protein phosphorylation in developing tomato fruits.  Plant Cell Physiology  26:  1565-1572.
    1. Poovaiah,  B.W. and K. Veluthambi.  1985.   Auxin-regulated invertase activity in strawberry fruits.  Journal of American Society for Horticultural Science  110: 258-261.
    1. Veluthambi,  K.,  J.K.  Rhee,  Y. Mizrahi  and B.W.  Poovaiah.  1985.  Correlation between lack of receptacle growth in response to auxin and accumulation of a specific polypeptide in a strawberry variant.  Plant Cell Physiology  26:  317-324.
    1. Veluthambi, K. and B.W.  Poovaiah.  1984.  Calcium- and calmodulin-regulated phosphorylation of soluble and membrane proteins from corn coleoptiles.  Plant Physiology 76:  359-365.
    1. Veluthambi, K. and B.W. Poovaiah.  1984.  Polyamine-stimulated phosphorylation of proteins from corn (Zea mays L.)   coleoptiles.  Biochemical and Biophysical Research Communications 122:  1374-1380.
    1. Giovanelli,  J.,  K. Veluthambi,  G. A. Thompson,  S.H. Mudd and A.H.  Datko.  1984.  Threonine synthase of Lemna  pauciscostata Hegelm.  6746.  Plant Physiolsogy  76:  285-292.
    1. Veluthambi,  K.,  and  B.W.  Poovaiah.  1984.  Calcium-promoted protein phosphorylation in plants.  Science  223:  167-169.
    1. Veluthambi,  K.  and B.W.  Poovaiah.  1984.  Auxin-regulated polypeptide changes at different stages of strawberry fruit development.  Plant Physiology  75:  349-353.
    1. Veluthambi,  K.,  S.  Mahadevan and R.  Maheshwari.  1982.  Trehalose toxicity in Cuscuta reflexa:  Cell wall synthesis inhibited upon trehalose feeding.  Plant Physiology  70:  686-688.
    1. Veluthambi, K., S. Mahadevan and R.  Maheshwari.  1982.  Trehalose toxicity in Cuscuta reflexa:   Sucrose content decreases in shoot tips upon trehalose feeding.  Plant Physiology 69: 1247-1251.
    1. Veluthambi,  K.,  S.  Mahadevan and R. Maheshwari.  1981.  Trehalose toxicity in Cuscuta reflexa:  Correlation with low trehalase activity.  Plant Physiology  68: 1369-1374.
    1. Maheshwari,  R.,  C.  Shailini,  K. Veluthambi and S.  Mahadevan.  1980.  The interaction of gibberellic acid and indole-acetic acid on the growth of excised Cuscuta shoot tips in vitro.  Plant Physiology  65:  186-192.

    BOOK ARTICLES

    1.      Poovaiah,  B.W.  and K. Veluthambi.  1986.  The role of calcium  and calmodulin in hormone action in plants:  Importance of protein phosphorylation.  In,   Molecular and Cellular Aspects of Calcium in Plant Development,  Ed,  A.J.  Trewavas.  Plenum Publishers,  Newyork.  pp 83-90

    2.      Veluthambi,  K.,  R.K.  Jayaswal and S.B. Gelvin.  1987.  Role of vir genes in the excision of T-DNA from the Ti plasmid.  In,  Molecular Genetics of Plant-Microbe Interactions,  Eds,  D.P.S. Verma and B.  Brisson.   Martinus  Nijhoff Publishers.  Boston.  pp  319-324.

    3.      Joon,  M.J.,  K. Veluthambi,  S.B.  Gelvin and W.  Ream.  1988.  The                      A.  tumefaciens  T-DNA transmission enhancer,  overdrive,  stimulates   T-strand accumulation.  In,  Physiology and Biochemistry  of Plant Microbe Interactions,  Eds.  N.T.  Keen,  T.  Kusuge,  L.L.  Waling.  The American Society of Plant Physiologists.  pp. 11-18.

    4.      Dharmalingam, K.  and K. Veluthambi.  1996.  Plant Biotechnology.  In,  Concepts in Biotechnology,   Eds,  D. Balasubramnian  et al.   COSTED  publishers,  Madras. pp. 252-304

    5.      Ramanathan,  Vai. and K. Veluthambi.  1997.  Transfer of Agrobacterium T-DNA         from   left   border  repeats.    In   Plant   Molecular   Biology   and Biotechnology,   K. K.  Tewari  and  G .S. Singhal  (Eds),   pp.  185-195.  Narosa    Publishing      House,  New Delhi.

    6.      Usha, R. and K. Veluthambi.  2003.  Genetic engineering for virus resistance.  In Improvement Strategies for Leguminosae Biotechnology, Eds, P.K. Jaiwal and R.P. Singh.  Kluwer Academic Publishers, Great Britain.  pp. 255-268.




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