Agrobacterium T-DNA transfer and improvement of Ti plasmid vector systems: 'Long
transfer' effects in Agrobacterium Ti plasmid vectors and vir gene induction by
rice have been demonstrated.
- Study of Mechanism of
and analysis of Mungbean yellow mosaic virus:- Cloning and sequencing of the
virus have been achieved. Transgenic rice plants generated with Chitinase, B-1,
3-glucanase, osmotin and thaumatin - like protein genes exhibit high levels of
sheath blight resistance.
engineering of crop plants for fungal and virus resistance.
Major ongoing research programmes in the lab are:
RESEARCH WORK CARRIED OUT DURING 2000-2005
- Genetic Engineering of rice for sheath resistance
- Molecular Biology of Mungbean Yellow mosaic virus Vigna(MYMV-Vig)
and Genetic Engineering of blackgram for MYMV-Vig resistance.
- Genetic Engineering of coffee for leaf rust resistance.
- Development of Ti plasmid vector for selection marker elimination in
MAJOR RESEARCH RECOGNITIONS DURING 2000-2005
- Elected Fellow of Indian Academy of Sciences(2002)
- Elected Member-Guha Research Conference
PLAN FOR RESEARCH WORK DURING 2005 - 2010
- Greenhouse and Field trials ot Transgenic rice with sheath blight
- Genetics Engineering of Blackgram for MYMV-Vig resistance.
- Rice functional genomics - 'Gene knock out' in rice.
- Marker-free transgenic rice-Technology Development.
RESEARCH PUBLICATIONS (1994 � 2005)
Shivaprasad, P.V., R. Akbergenov, D. Trinks,
R. Rajeswaran, K. Veluthambi,
T. Hohn and M. M. Pooggin. 2005. Promoters, transcripts and regulatory
proteins of mungbean yellow mosaic geminivirus. J. Virology 79: 8149-8160.
Guerra-Peraza, O., D. Kirk, V. Seltzer,
A.C. Schmidt, T. Hohn and E. Herzog. 2005. Coat proteins of Rice tungro
bacilliform virus and Mungbean yellow mosaic virus contain multiple nuclear-lacalization
signals and interact with importin a.
J. General Virology 86: 1815-1826.
Trinks, D., R. Rajeswaran, P. V. Shivaprasad,
R. Akbergenov, E.J. Oakeley, K.
Veluthambi, T. Hohn and M.M.
Pooggin. 2005. Suppression of RNA silencing by a geminivirus nuclear protein,
AC2, correlates with transactivation of host genes. J. Virology 79:
Sridevi G., M.Dhandapani and
2005. Agrobacterium-mediated transformation of White Ponni, a non-basmati
variety of indica rice (Oryza sativa L.). Current Science 88: 128-132.
Karthikeyan, A.S., R. Vanitharani, V. Balaji,
S. Anuradha, P. Thillaichidambaram, P. V. Shivaprasad, C. Parameswari, V.
Balamani, M. Saminathan and K.
Veluthambi. 2004. Analysis of an
isolate of Mungbean yellow mosaic virus (MYMV) with a highly variable DNA B
component. Archives of Virology 149: 1643-1652.
Balaji, V., R. Vanitharani, A.S. Karthikeyan,
S. Anbalagan and K.Veluthambi.
2004. Infectivity analysis of two variable DNA B components of Mungbean
yellow mosai virus �Vigna in Vigna mungo and Vigna radiata. Journal of
Biosciences 29: 297-308.
Sridevi, G., N. Sabapathi, P. Meena, R.
Nandakumar, R. Samiyappan, S. Muthukrishnan, and
2003. Transgenic indica rice variety Pusa Basmati 1 constitutively expressing
a rice chitinase gene exhibits enhanced resistance to Rhizoctonia solani. J.
Plant Biochem & Biotechnol 12: 93-101.
Jacob, S.S., R. Vanitharani, A.S. Karthikeyan,
Y. Chinchore, P. Thillaichidambaram, and
2003. Mungbean yellow mosaic virus-Vi agroinfection by codelivery of DNA A
and DNA B from one Agrobacterium strain. Plant Disease 87: 247-251.
Mikhail Pooggin, P.V. Shivaprasad,
and Thomas Hohn. 2003. RNAi targeting of DNA virus in plants. Nature
Biotechnology 21: 131-132.
Aditya K. Gupta and Aurn Sharma. 2003. The Current status of plant
transformation technologies. Current Science 84: 368-380.
V. Balaji, P. Rajamuni, G. Sridevi and
2003. Agrobacterium-mediated Transformation Efficiency in Blackgram and Rice
Enhanced by Multiple Copies of pTiBo542 virB and virG. Indian Journal of
Biotechnology 2: 138-146.
Subha S. Jacob and
2003. A Cointegrate Ti Plasmid Vector for Agrobacterium tumefaciens-mediated
Transformation of indica Rice cv Pusa Basmati 1. J. Plant Biochemistry &
Biotechnology 12: 01-09.
Jacob, S.S. and
2002. Generation of selection marker-free transgenic plants by
cotransformation of a cointegrate vector T-DNA and a binary vector T-DNA in
one Agrobacterium tumefaciens strain. Plant Science 163: 801-806.
Atmakuri Krishnamohan, V. Balaji and
2001. Efficient vir gene induction in Agrobacterium tumefaciens requires
virA, virG and vir box from the same Ti plasmid. Journal of Bacteriology
Saunders, K., C. Wege,
H. Jeske and J. Stanley (2001). The distinct phenotypes of the common
and yellow vein strains of Tomato golden mosaic virus are determined
by nucleotide differences in the 3�-terminal region of the gene encoding the
movement protein. Journal of General Virology 82: 45-51.
Phogat, S. K., A. S. Karthikeyan
and K. Veluthambi
(1999). Generation of transformed calli of Vigna radiata (L.)
Wilczek by Agrobacterium tumefaciens-mediated transformation. Journal of
Plant Biology 26: 77-82.
Sunilkumar, G. K. Vijayachandra and
1999. Preincubation of cut tobacco leaf explants promotes Agrobacterium-mediated
transformation by increasing vir gene induction. Plant Science 141: 51-58.
Madhava Naidu, M.
C. S. Srinivasan and R. Naidu. 1998. Agrobacterium-mediated transformation
in Coffea canephora. Developments in plantation crops Research pp. 46-50.
Mariamma, M., B. Muthukumar,
K. Veluthambi and A. Gnanam.
1997. Effects of high temperature stress on the expression of low molecular
weight heat shock proteins in rice leaves. Journal of Plant Physiology 151:
Muthukumar, B., M. Mariamma,
and A. Gnanam. 1996. Genetic transformation of cotyledon explants of cowpea
(Vigna unguiculata L. Walp) using Agrobacterium tumefaciens. Plant Cell
Reports 15: 980-985.
Palanichelvam, K. and
1996. Octopine- and nopaline-induced proteins in Agrobacterium tumefaciens
are also induced by arginine. Current Microbiology 33: 156-162.
Ramanathan, Vai. and
1996. Analysis of Octopine left border-directed transfer from
Agrobacterium to plants. Journal of Biosciences 21: 45-57.
Karthikeyan, A.S., K. S. Sarma and
1996. Agrobacterium tumefaciens-mediated transformation of Vigna mungo (L.)
Hepper. Plant Cell Reports 15: 328-331.
Vanitha Rani, R., A.S. Karthikeyan, S.
Anuradha and K. Veluthambi.
1996. Genome homologies among geminiviruses infecting Vigna, cassava,
Acalypha, Croton amd Vernonia. Current Science (India) 70: 63-69
Sarma, K.S., G. Sunil Kumar, V. Balamani
and K. Veluthambi.
1995. Transient b-glucuronidase
expression and generation of shoot buds are highly correlated in Agrobacterium
transformed tobacco leaf rings. Plant Molecular Biology Reporter 13:
Vijayachandra, K., K. Palanichelvam, and
1995. Rice scutellum induces Agrobacterium tumefaciens vir genes and
T-strand generation. Plant Molecular Biology 29: 125-133.
Ramanathan, Vai. and
1995. Transfer of non T-DNA portions of Agrobacterium Ti plasmid pTiA6 from
the left terminus of TL-DNA. Plant Molecular Biology 28: 1149-1154.
M. Krishnan, J.H. Gould, R.H. Smith and S.B. Gelvin. 1989. Opines
stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti
plasmid. Journal of Bacteriology 171: 3696-3703.
W. Ream and S.B. Gelvin. 1988. Virulence genes, borders and overdrive
generate single-stranded T-DNA molecules from the A6 Ti plasmid of
Agrobacterium tumefaciens. Journal of Bacteriology 170: 1523-1532.
S.B. Gelvin and J.L. Slightom. 1987. Double-stranded cleavage of T-DNA and
generation of single-stranded T-DNA molecules in Escherichia coli by a
virD-encoded border-specific endonuclease from Agrobacterium tumefaciens.
Journal of Bacteriology 169: 5035-5045.
R.K. Jayaswal and S.B. Gelvin. 1987. Virulence genes A, G and D mediate the
double-stranded border cleavage of T-DNA from the Agrobacterium Ti plasmid.
Proceedings of the National Academy of Sciences USA 84: 1881-1885.
and B.W. Poovaiah. 1986. In vitro and in
vivo protein phosphorylation in Avena sativa L. coleoptiles:
Effects of Ca2+, calmodulin antogonists and auxin. Plant Physiology 81:
and B.W. Poovaiah. 1986. Role of calcium in the control of tuberization in
potato (Solanum tuberosum L.). Plant Physiology 80: 856-858.
and B.W. Poovaiah. 1985. Stage-specific changes in calcim-regulated protein
phosphorylation in developing tomato fruits. Plant Cell Physiology 26:
Poovaiah, B.W. and
1985. Auxin-regulated invertase activity in strawberry fruits. Journal of
American Society for Horticultural Science 110: 258-261.
J.K. Rhee, Y. Mizrahi and B.W. Poovaiah. 1985. Correlation between lack
of receptacle growth in response to auxin and accumulation of a specific
polypeptide in a strawberry variant. Plant Cell Physiology 26: 317-324.
and B.W. Poovaiah. 1984. Calcium- and calmodulin-regulated phosphorylation
of soluble and membrane proteins from corn coleoptiles. Plant Physiology 76:
and B.W. Poovaiah. 1984. Polyamine-stimulated phosphorylation of proteins
from corn (Zea mays L.) coleoptiles. Biochemical and Biophysical Research
Communications 122: 1374-1380.
K. Veluthambi, G. A. Thompson,
S.H. Mudd and A.H. Datko. 1984. Threonine synthase of Lemna pauciscostata
Hegelm. 6746. Plant Physiolsogy 76: 285-292.
and B.W. Poovaiah. 1984. Calcium-promoted protein phosphorylation in
plants. Science 223: 167-169.
and B.W. Poovaiah. 1984. Auxin-regulated polypeptide changes at different
stages of strawberry fruit development. Plant Physiology 75: 349-353.
S. Mahadevan and R. Maheshwari. 1982. Trehalose toxicity in Cuscuta
reflexa: Cell wall synthesis inhibited upon trehalose feeding. Plant
Physiology 70: 686-688.
S. Mahadevan and R. Maheshwari. 1982. Trehalose toxicity in Cuscuta reflexa:
Sucrose content decreases in shoot tips upon trehalose feeding. Plant
Physiology 69: 1247-1251.
S. Mahadevan and R. Maheshwari. 1981. Trehalose toxicity in Cuscuta reflexa:
Correlation with low trehalase activity. Plant Physiology 68: 1369-1374.
Maheshwari, R., C. Shailini,
K. Veluthambi and S. Mahadevan.
1980. The interaction of gibberellic acid and indole-acetic acid on the
growth of excised Cuscuta shoot tips in vitro. Plant Physiology 65:
Poovaiah, B.W. and K. Veluthambi. 1986. The role of calcium
and calmodulin in hormone action in plants: Importance of protein
phosphorylation. In, Molecular and Cellular Aspects of Calcium in Plant
Development, Ed, A.J. Trewavas. Plenum Publishers, Newyork. pp 83-90
R.K. Jayaswal and S.B. Gelvin. 1987. Role of vir genes in the excision of
T-DNA from the Ti plasmid. In, Molecular Genetics of Plant-Microbe
Interactions, Eds, D.P.S. Verma and B. Brisson. Martinus Nijhoff
Publishers. Boston. pp 319-324.
Joon, M.J., K. Veluthambi, S.B. Gelvin and W. Ream. 1988.
The A. tumefaciens T-DNA transmission enhancer,
overdrive, stimulates T-strand accumulation. In, Physiology and
Biochemistry of Plant Microbe Interactions, Eds. N.T. Keen, T. Kusuge,
L.L. Waling. The American Society of Plant Physiologists. pp. 11-18.
Dharmalingam, K. and K. Veluthambi. 1996. Plant Biotechnology.
In, Concepts in Biotechnology, Eds, D. Balasubramnian et al. COSTED
publishers, Madras. pp. 252-304
Ramanathan, Vai. and K. Veluthambi. 1997. Transfer of
Agrobacterium T-DNA from left border repeats. In Plant
Molecular Biology and Biotechnology, K. K. Tewari and G .S. Singhal
(Eds), pp. 185-195. Narosa Publishing House, New Delhi.
Usha, R. and K. Veluthambi. 2003. Genetic engineering for virus
resistance. In Improvement Strategies for Leguminosae Biotechnology, Eds, P.K.
Jaiwal and R.P. Singh. Kluwer Academic Publishers, Great Britain. pp. 255-268.